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. 2014 May 7;71(18):3507–3521. doi: 10.1007/s00018-014-1634-z

Table 1.

Secondary structure assignments of Aβ fibrils and structures deposited in the PDB

Peptide Flexible regions (solvent-exposed) β-structured regions (non-exposed) Method References
Aβ1−40

N-terminus (Asp1-Phe19)

C-terminus (Met35-Val40)

 Phe20-Leu34 HDX-MS coupled with online proteolysis [191]
Aβ1−40

N-terminus (Asp1-His14)

C-terminus (Gly37-Val39)

Turn? (Ser26-Asn27)

Gln15-Asp23

Lys28-Met35

HDX-solution NMR [52]
Aβ1−40

N-terminus (Asp1-His14)

C-terminus (Gly37-Val40)

Turns (Glu22-Asp23, Gly29-Ala30)

Gln15-Ala21

Val24-Lys28

Ile31-Val36

Scanning proline mutagenesis [58]
Aβ1−40

N-terminus (Asp1-Tyr10)

Bend (Gly25-Gly29)

Val12-Val24

C-terminus (Ala30-Val40)

 Solid-state NMR [50]
Aβ1−40

N-terminus (Asp1-Gly9)

Bend/loop (Asp23-Gly29)

Tyr10-Glu22

C-terminus (Ala30-Val40)

 Solid-state NMR [59]
Aβ1−40 Aβ1−42

N-terminus (Asp1-Tyr10)

C-terminus (Val40-Ala42?)

Turn/bend? (Asp23-Gly29)

 His14-Gly38 Site-directed spin labeling-EPR [44]
Aβ1−42

N-terminus (Asp1-Leu17)

Turn (Asn27-Ala30)

Val18-Ser26

C-terminus (Ile31-Ala42)

 HDX-solution NMR [53]
Aβ1−42

N-terminus (Asp1-Tyr10)

Bend region? (Ser26-Asn27)

Glu11-Gly25

C-terminus (Lys28-Ala42)

 HDX-solution NMR [54]

Fibril structures deposited in the PDB: synthetic Aβ1−40 (2LMN, 2LMO, 2LMP, 2LMQ), brain-derived Aβ1−40 (2M4J), synthetic D23N Aβ1−40 (2LNQ), recombinant Aβ1−42 (2BEG)