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. 2014 May 20;26(3):255–273. doi: 10.1007/s12640-014-9477-9

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© The Author(s) 2014

Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Fig. 1 — Microphotographs from MAP-2 immunofluorescence of 7DIV mouse primary cortical neurons treated with celastrol (Cel, 1 μM) and lactacystin (Lac, 2.5 μg/ml) for 48 h (a, d, e, and f). Celastrol enhanced the reduction in the number of MAP-2 positive cells in lactacystin-treated primary neuronal cell cultures. The purity of neuronal cell cultures (about 90 % neurons) was confirmed by double-immunostainig of vehicle-treated cells with neuronal (anti-MAP-2) and glia (anti-GFAP) specific markers (a, b, and c)