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. 2014 Mar 27;76(7):1055–1059. doi: 10.1292/jvms.13-0625

Table 2. PCR primers. Restriction sites used for cloning are underlined.

Primers Sequence (5′-3′) Description
SSU0587-1F TTAGAATTCAGACCCTGCTGGCTATTA 5′ SSU0587 flanking, EcoRI
SSU0587-1R TATCCTGTATGCATTATTTCCTCCTATAAAGT 5′ SSU0587 region, EcoT22I
SSU0587-2F AGGAAATAATGCATACAGGATAGTGAGTACTT 3′ SSU0587 region, EcoT22I
SSU0587-2R CGACTGCAGTATTAAGAATTTTATGCC 5′ SSU0587 flanking, PstI
TsrtA-1F ATAGAATTCAACAAATGGCCAAGCAGACA 5′ srtA flanking, EcoRI
TsrtA-1R TAGATGTTATGCATGACTATTTATTCCTTTTCTT 5′ srtA region, EcoT22I
TsrtA-2F AAATAGTCATGCATAACATCTATATATTAAGGAT 3′ srtA region, EcoT22I
TsrtA-2R TATAAGCTTTACGTCCCAACTTCTCACTT 5′ srtA flanking, HindIII
6s-F TAGTATGCATTAATTCGATGGGTTCCGAGG 5′ end of cat, EcoT221
6s-R TCACATGCATCACCGAACTAGAGCTTGATG 3′ end of cat, EcoT221
SSU0587-F TGGGGACGGGGGCATTAT 5′ SSU0587 segment
SSU0587-R GCGCTTTAGAGTTCTTCGGAGTT 3′ SSU0587 segment
srtA-F CAAGAGATGGGGAAAGGAAA 5′ srtA segment
srtA-R TGTCCATAATCATACTGATT 3′ srtA segment
spc-F GTTCGTGAATACATGTTATA 5′ spc segment
spc-R GTTTTCTAAAATCTGAT 3′ spc segment