Table 1. Primers used for real-time PCR.
Target * | Sequence (5’-3’) | Reference |
---|---|---|
SAA1/2 F | CTGCCTGCCAAATACTGAGAGTC | Eckhardt et al., 2010 [2] |
SAA1/2 R | CCACTTCCAAGTTCCTGTTTATTAC | |
SAA3 F | GCTGGCCTGCCTAAAAGATACTG | Eckhardt et al., 2010 [2] |
SAA3 R | GCATTTCACAAGTATTTATTCAGC | |
IL-1β F | CCCAAGCAATACCCAAAGAA | Li et al., 2009 [8] |
IL-1β R | CATCAGAGGCAAGGAGGAAA | |
IL-6 F | TTCCATCCAGTTGCCTTCTT | Yang et al., 2006 [31] |
IL-6 R | ATTTCCACGATTTCCCAGAG | |
TNF-α F | GCCTCTTCTCATTCCTGCTT | Yang et al., 2006 [31] |
TNF-α R | CACTTGGTGGTTTGCTAGGA | |
MUC2 F | GCTGACGAGTGGTTGGTGAATG | Wlodarska et al., 2011 [30] |
MUC2 R | GATGAGGTGGCAGACAGGAGAC | |
GAPDH F | TGCACCACCACCAACTGCTTAG | This study |
GAPDH R | GGATGCAGGGATGATGTTC | Accession No. GU214026 |
*SAA, serum amyloid A; IL, interleukin; TNF-α, tumor necrosis factor-α; MUC2, mucin 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.