Table 2. Genetic linkage between HaABCC2 and resistance to Cry1Ac.
| Larvae with insertion (%) | ||
|---|---|---|
| Backcross family | Control diet | Treated diet |
| A1 | 47 | 100 |
| A2 | 45 | 100 |
| A3 | 53 | 100 |
| A4 | 50 | 100 |
| A5 | 52 | 100 |
| B6 | 52 | 100 |
| B7 | 43 | 100 |
| B8 | 52 | 100 |
| B9 | 40 | 100 |
| B10 | 50 | 100 |
| Mean | 48 | 100 |
Backcross families A1–A5 were generated by crossing a resistant male with an F1 female and families B6–B10 by crossing an F1 male with a resistant female. For each of the 10 backcross families, genotype was determined for 120 larvae (60 fed control diet and 60 fed diet treated with Cry1Ac). Genomic DNA sequencing method was used to determine genotype. The proportion of larvae with the insertion was significantly higher on treated than control diet (Fisher's exact test, P < 0.0001 for each family). On control diet, the proportion of larvae with the insertion did not differ significantly from the expected proportion of 0.5 (P > 0.35 for each family).