Table 1.

NAD(P)H reduction and predicted rates of superoxide production by site I_F in Drosophila mitochondria.

Substrates and inhibitors	NAD(P)H reduction(% of maximum)	Predicted rate of superoxide
		Production from site IF
		(pmol min−1 mg protein−1)
Proline (Fig. 3a)	5.5±1.9	11.0±1.0
Proline, malonate (Fig. 3a)	2.6±1.8	10.3±0.6
Proline, rotenone (Fig. 3b)	98.0±1.0	67.8±14.7
Proline, myxothiazol (Fig. 3b)	37.9±5.6	20.8±8.7
Proline, myxothiazol, malonate (Fig. 3b)	20.8±4.9**	17.7±5.8
Proline, myxothiazol, rotenone, A5, malonate (Fig. 3b)	100	70.4±15.4

^⁎⁎p=0.007 comparing proline, myxothiazol and proline, myxothiazol and malonate (paired t-test, one-tailed).