Table 2.

NAD(P)H reduction and predicted rates of superoxide production by site I_F in ZR75-30 mitochondria.

Substrates and inhibitors	NAD(P)H reduction	Predicted rate of superoxide
	(% of maximum)	production from site IF
		(pmol min−1 mg protein−1)
Proline	59.5 ± 8.0	24.9 ± 2.8
Proline, aspartate and ATP	60.0 ± 5.0	25.0 ± 2.65
Proline, rotenone, myxothiazol, malonate, A5	51.9 ± 1.4	23.8 ± 2.1
Proline, rotenone, myxothiazol, malonate, A5, Aspartate and ATP,	41.3 ± 3.9*	22.8 ± 1.8

Conditions are described in Fig. 4a.

^⁎p=0.04 vs proline, rotenone, myxthiazol, malonate and A5 (paired t-test, one-tailed).