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nSMase mRNA in skeletal muscle. (A) Real-time PCR of known nSMases in human and mouse diaphragm. (B) Real-time PCR of nSMase3 mRNA in different mouse skeletal muscles and myotubes (AU=arbitrary units, n=13 for human tissues, n=4 for mouse, n=3 for myotubes). Abundances are normalized to rpl13A. ⁎P<0.0001 by ANOVA.
