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nSMase3 splice variant activity. (A) Fluorescence images of GFPnSMase3a and GFPnSMase3b in myotubes (scale bar=100 µm). (B) Western blot of lysates transfected with GFPnSMase3a or b using anti-nSMase3. (C) Quantification of B (n=3), P<0.001 vs. GFPnSMase3a. (D) nSMase activity in lysates from myotubes transfected with GFPnSMase3a or GFPnSMase3b (choline release, n=6), ⁎P<0.05 vs. GFP control.
