Fig. 9.

nSMase3a and oxidant signaling. (A) nSMase activity in myotubes transfected with control or siRNA to nSMase3a (exon 11, 20 nM)±TNF (6 ng/ml, 5 min, n=4, ceramide release assay, P<0.002 by ANOVA, ^⁎ different from untreated, ^#different from TNF-treated control siRNA by Bonferroni’s multiple comparison). (B) Oxidant activity in myotubes transfected with control or siRNA to nSMase3a (as in A)±TNF (6 ng/ml, 20 min, n=9 per group, ^⁎P<0.001, paired comparisons). Oxidant activity analysis was performed as in Fig. 7B.