Table 1.
Oligonucleotide primers.
| Real time PCR | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
| Mouse | ||
| nSMase1 | CTCGCCGCCCTTGCT | CAGCCGTAGAGAAAAGTTGAGCTT |
| nSmase2 | CACCAACACCTCCATCAGTG | GTCAGCCTGTTCTCCAGAGG |
| nSMase3 | CCCTACGCACAGACCTTGTCA | AAGACCTTAGCCACTCGGAAGA |
| nSMase3a | ACCGACTCACTGTCTCCAGC | GACTCTTGGTAGGCGTGGAG |
| nSMase3b | CTCATGCCAAGGAGTCCTTC | AAGGCATGCAGGTGCTTC |
| nSMase-MT | TCATGGGAGTTACCCAGCTC | CGACATTCATCCCCTTGTCT |
| rpl13A | GCAAGTTCACAGAGGTCCTCAA | GGCATGAGGCAAACAGTCTTTA |
| Human | ||
| nSMase1 | TTTGGTGTCCGCATTGACTA | TAGAGCTGGGGTTCTGCTGT |
| nSMase2 | GGAAGGCCGAGGTGGAA | CCCCCGAAGACACCATCA |
| nSMase3 | CACCCAGGATGAGAATGGAAA | GTCCGTCCTCACCCACGAT |
| nSMase-MT | GAGCTAAGCCCTGGAGAGGT | GACACTGAGACTCGGAGCTG |
| rpl13A | TCCTGGTCTGAGCCCAATAAA | CAGGGCAACAATGGAGGAA |
| Conventional PCR | Forward (5′–3′) | Reverse (5′–3′) |
| Human nSMase3 | ||
| Exons 7–12 | TCTTTGCCTTGAGCCTCATC | ACCACCAACACATGCTCCTC |
| Exons 10–13 | TGTTGAAATGTGGCTTCATCA | GAGTTTCTGCTGGACGAACC |
| Mouse nSMase3 | ||
| Exons 9–13 | TTTGTGGAAATGTGGCTTCA | CCTGTTTCTCAGGGGCATAC |
| Cloning primers | Forward (5′–3′) | Reverse (5′–3′) |
| ccgctcgagCTATGGCGTTCCCTCAC | cgggatccTCAGAGCTGGTGCAGCTT | |
| Morpholino oligos | Antisense | |
| nSMase2 | ACAAAACCATTGCAGCTCATGGGC | |
| nSMase3 | TGAGGGAACGCCATAGCAGACTCCC | |
| siRNA oligos | Sense | Antisense |
| nSmase3 exon 11 | GAGGUUCUACACUACCGACtt | GUCGGUAGUGUAGAACCUCca |
| nSmase3 exon 18 | GAUCAUUAAUGGUCUGCGAtt | UCGCAGACCAUUAAUGAUCtg |