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. Author manuscript; available in PMC: 2014 Aug 26.
Published in final edited form as: J Drug Target. 2012 Sep;20(8):678–690. doi: 10.3109/1061186X.2012.712128

Figure 4.

Figure 4

(A) TAT-SA wt and mt genetic constructs. TAT sequence is inserted at the NdeI site at the N-terminus of the SA gene. The NdeI site is preserved at the N-terminus of the fusion gene after the insertion. The yellow triangles indicate three amino acid mutations in the TAT-SA mt gene. A BamHI site is interrupted by one of the mutations and used in diagnostic digestion to verify the identity of the two genes. TAT reverse oligo and an upstream sequencing primer are used for colony PCR. (B,C) SDS-PAGE of SA and TAT-SA. Samples were loaded onto 15% SDS-PAGE gels with or without boiling. (B) Different TAT-SA tetramers eluted from the Ni-NTA column with imidazole of the concentrations indicated in the figure. (C) SA and TAT-SA after one month storage at 4°C or −80°C.