Figure 1. Blocks C and A are necessary and sufficient for FT expression in the leaf.

(a) Schematic representation of different FT promoter constructs used in the analysis (TSS is +1). (b) Flowering time of ft-10 plants transformed with FT cDNA constructs driven by different FT promoters as depicted in a. Numbers of rosette and cauline leaves are shown as mean±s.e. Statistical significance was determined using one-way analysis of variance (ANOVA) and multiple comparison by the Holm–Sidak method (P<0.01). Different Greek symbols above the bars indicate significant differences. (c) Histochemical localization of GUS activity in the first true leaves of transgenic Col-0 expressing GUS gene under the control of different FT promoters as depicted in a. Leaves were collected at ZT16 after growth for 12 LDs on GM media. (d) Reverse transcriptase (RT)-qPCR analysis of FT in ft-10 seedlings carrying the 5.7 kb, 5.2 kb or dMp promoter fused to FT cDNA. Seedlings were harvested from ZT0 to ZT24 on day 10 in LDs. Values represent mean±s.e. of FT relative to PP2A levels for three technical replicates. A biological replicate showed similar results. (e) RT-qPCR of FT in ft-10 seedlings carrying the 5.7 kbp, C+Ap or dMp promoter fused to FT cDNA. Seedlings were harvested at ZT 16 on day 11 in LD conditions. Values in d and e represent mean±s.e. of relative FT to PP2A levels for three technical replicates. A biological replicate showed similar results.