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. 2014 Aug 14;5:4657. doi: 10.1038/ncomms5657

Figure 7. RasGRP3 inhibits TLR-triggered IL-6 production in vivo.

Figure 7

(a–d) Wild-type mice, RasGRP3 or RasGRP3-T133A transgenic mice were injected with indicated amounts of LPS (a,b), Poly (I:C) (c) or CpG ODN (d) intraperitoneally. Two and a half hours later, IL-6 levels in serum were determined by ELISA. (e–g) Irradiated wild-type mice were intraperitoneally injected with wild-type, RasGRP3 or RasGRP3-T133A transgenic peritoneal macrophages 8 days after bone marrow transplantation. Two days after macrophages transfer, LPS (e), Poly (I:C) (f) or CpG ODN (g) were intraperitoneally injected. Two and a half hours later, IL-6 levels in serum were determined by ELISA. Data are presented as mean±s.d. (n=11 in a, n=12 in b, n=11 in c, n=9 in d, and n=5 in e,f and g). All experiments were repeated three times. **P<0.01; ***P<0.001 (Mann–Whitney U-test or analysis of variance, as compared with wild-type group).