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. 2014 Aug 19;5:4710. doi: 10.1038/ncomms5710

Figure 3. VLA-4 plays a critical role in transmigration of iMo into the peritoneal cavity after CLP.

Figure 3

(a) Mice were treated with control antibody, anti-VLA-4 mAb or anti-LFA-1 mAb immediately before CLP. Numbers of Mφ/Mo (CD11b+ F4/80+) and neutrophils (CD11b+ F4/80− Ly6G+) in the peritoneal cavity 20 h after CLP operation (n=6–9 per group). (b) CFSE-labelled iMo from WT or AF251705−/− mice were adoptively transferred intravenously into AF251705−/− mice. These mice were treated with anti-VLA-4 mAb or control antibody immediately before CLP. Migrated CFSE+ iMo in the peritoneal cavity were counted by using flow cytometry 20 h after CLP (n=4 or 5 per group). N.S., not significant. *P<0.05 and ***P<0.001 (two-tailed Student’s t-test in a,b). Error bars indicate s.d. Data are representative of two independent experiments.