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. 2014 Jul 27;4(10):972–989. doi: 10.7150/thno.9436

Figure 1.

Figure 1

Generation of a transgenic mESC cell line expressing inducible magA. (A) Schematic illustration of LV-Tet-MagA: HA-tagged magA under the control of TRE was expressed in a modified FUGW vector. Zeocin was expressed through the IRES downstream of the ubiquitin promoter. (B) Quantitative real-time PCR (qPCR) analysis of magA expression in mESC-MagA demonstrates a positive correlation between the expression of magA and dosage of Dox. Maximum magA expression was observed when supplemented with 1 µg/ml of Dox (9.74 ± 2.49, **P < 0.001, n = 3). (C) Western blot analysis of MagA assessed via HA tag in mESC-MagA demonstrates no differences when supplemented with different dosages of Dox (0.25, 0.5, 1, and 2 µg/ml) as shown in densitometry analysis. (D) Expression of MagA was confirmed by immunofluorescence using HA-specific antibody (yellow). The nucleus was visualized by Hoechst 33342 (blue). All histogram data are means ± SEM. *P< 0.05, **P< 0.001 versus appropriate control by ANOVA.