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. 2014 Jul 27;4(10):972–989. doi: 10.7150/thno.9436

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Evaluation of pluripotency of mESC-MagA. (A) mESC-WT and mESC-MagA expressing pluripotency markers: Nanog, Sox2, and Oct4. Nucleus is visualized with Hoechst 33342. (B) Teratomas that developed from subcutaneous injection of 1x10⁶mESC-WT and mESC-MagA cells into a nude mouse shoulder subsequently developed all three germ layers: ectoderm (neural epithelium), endoderm (ciliated epithelium and glands), and mesoderm (striated muscle). Also, Prussian blue-positive cells were observed (indicated by arrow heads) in mESC-MagA teratoma.