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. 2014 Jul 27;4(10):972–989. doi: 10.7150/thno.9436

Figure 9.

Figure 9

Confirmation and correlation of MagA expression and iron content in teratoma. (A) Immunohistochemical DAB staining using HA antibody demonstrated MagA expression throughout mESC-MagA tumor. (B) At higher magnification, images of different brain regions (1-mESC-WT, 2-mESC-MagA, and 3-Brain) demonstrated distinct intensity, which was also revealed by three-dimensional image of the brain section. (C) Immunofluorescence analysis using HA antibody demonstrated MagA expression in mESC-MagA-derived teratoma. A clear boundary was visible, and MagA expression was only detectable in teratoma developed from mESC-MagA transplantation. (D) Quantitative real-time PCR measurement demonstrates the expression of magA seen only in teratoma developed from mESC-MagA transplantation, while there was no expression in teratoma samples developed from mESC-WT (**P< 0.001, n = 4). (E) A representative Prussian blue staining of a brain section demonstrated higher frequency of Prussian blue-positive cells in mESC-MagA (3 and 4) derived teratoma compared to mESC-WT side (1 and 2). All histogram data are means ± SEM. *P< 0.05, **P< 0.001 versus appropriate control by ANOVA.