Fig. 2.

Nonhost pepper shares expression profiles of the effector genes of Phytophthora infestans with host plants. Detached leaves of pepper (Capsicum annuum) and tomato (Solanum lycopersicum) upon inoculation with P. infestans isolate 43072 were harvested at 0, 12 h, and 1, 2 and 3 days post-inoculation. Total RNA was extracted from the harvested samples. Reverse transcription-PCR was performed to observe in planta expression of PexRD effector genes. Tomato cDNAs was subjected to a 30-cycle PCR and a 35-cycle PCR was performed with pepper cDNAs. Constitutive ActA and in planta-induced EPI1 were used as controls and those genes were detected using 30-cycle PCR in both pepper and tomato. This experiment was repeated twice with similar results. M, P. infestans mycelium RNA; G, P. infestans genomic DNA. *PexRD2 and PexRD8 in tomato cDNAs: subjected to a 35-cycle PCR.