Skip to main content
. 2014 Aug 6;5:4602. doi: 10.1038/ncomms5602

Figure 1. Normal T-cell development in T-HGK cKO mice.

Figure 1

(a) Schematic diagram of the mouse HGK WT allele, the gene targeting vector and the targeted HGK floxed allele. (b) T-cell-specific deletion of HGK in T-HGK cKO mice. T cells were purified from the spleen of mice. The remaining cells (non-T cells) were studied as a control. The expression of HGK and tubulin in T cells and non T cells was determined by immunoblotting analyses. (c,d) Flow cytometry analyses of T cells (c) and Treg cells (d) from the thymus and spleen of 5-week-old WT and T-HGK cKO mice. WT, littermate controls (HGKf/f mice or CD4-Cre mice); HGK cKO, T-HGK cKO mice. Data shown are representatives of three independent experiments.