Figure 7. IL-6/IL-17 double-positive T cells are pathogenic.
ELISA assay of fasting (a) IL-6/IL-17, (b) insulin and (c) glucose levels in the sera of indicated recipients 14 days after adoptive transfer. n=3 in (a); n, at least 5 (b,c). (d) GTT tests were performed on indicated recipients at 14 days after adoptive transfer. n, at least 5. (e,f) GTT tests of 12-week-old mice at day 2 after IL-6 (e) or IL-17 (f) neutralization. Glucose levels were plotted versus time post glucose injection. n=6 per group. Data shown are representatives of two independent experiments. Means±s.e.m. are shown. WT, littermate controls (HGKf/f mice or CD4-Cre mice); HGK cKO, T-HGK cKO mice. *P-value<0.05; **P-value<0.01 (two-tailed Mann–Whitney U-test).