Table 3.

Values of repeatability, within-laboratory reproducibility and inter-laboratory reproducibility obtained during the validation of the method for quantification of various phytohormones (ABA, IAA, JA-Ile, SA, JA, and OPDA) in Arabidopsis thaliana.

	Expected conc. (ng/g FW)*	Repeatability n = 3			Within laboratory reproducibility n = 6			Inter-laboratory reproducibility n = 9
		Mean ± SD	RSD (%)	Error (%)	Mean ± SD	RSD (%)	Error (%)	Mean ± SD	RSD (%)	Error (%)
ABA	7.00	6.67 ± 0.59	8.91	−4.73	6.92 ± 0.56	8.11	−1.15	7.01 ± 0.31	4.37	0.21
	350.00	344.22 ± 11.30	3.28	−1.65	361.58 ± 26.76	7.40	3.31	359.20 ± 22.21	6.18	2.63
	1400.00	1331.25 ± 57.27	4.30	−4.91	1422.62 ± 144.62	10.17	1.58	1401.39 ± 122.85	8.77	0.10
IAA	7.00	7.12 ± 0.71	9.96	1.67	7.29 ± 0.47	6.43	4.09	7.36 ± 0.21	2.86	5.09
	350.00	313.07 ± 8.70	2.78	−10.55	326.16 ± 22.11	6.78	−6.81	332.73 ± 17.28	5.13	−4.94
	1400.00	1306.57 ± 63.12	4.83	−6.67	1378.70 ± 7.32	7.32	−1.52	1353.86 ± 105.62	7.80	−3.30
JA-Ile	1.40	1.57 ± 0.11	6.76	12.34	1.56 ± 0.10	6.36	11.76	1.54 ± 0.03	2.19	10.32
	70.00	70.30 ± 1.85	2.64	0.43	72.24 ± 4.02	5.56	3.20	71.04 ± 3.47	4.88	1.48
	280.00	273.76 ± 3.36	2.36	−2.23	290.30 ± 22.26	7.67	3.68	286.83 ± 22.10	7.71	2.44
SA	140.00	138.46 ± 16.48	11.9	−1.10	138.25 ± 11.59	8.38	−1.25	136.18 ± 3.54	2.60	−2.73
	1400.00	1367.10 ± 43.35	3.17	−2.35	1355.08 ± 84.25	6.22	−3.21	1321.77 ± 56.00	4.24	−5.59
	2800.00	2598.57 ± 112.39	4.32	−7.19	2667.01 ± 145.34	5.45	−4.75	2612.61 ± 139.18	5.33	−6.69
JA	70.00	73.62 ± 3.79	5.15	5.18	70.87 ± 5.63	7.95	−1.25	69.83 ± 3.36	4.82	−0.25
	700.00	691.744 ± 76.12	11.00	−1.18	690.70 ± 63.21	9.15	−1.33	674.38 ± 27.99	4.15	−3.66
	1400.00	1348.90 ± 59.33	4.40	−3.65	1371.20 ± 107.43	7.83	−2.06	1354.27 ± 44.22	3.27	−3.27
OPDA	225.00	220 ± 12.11	5.48	−1.78	233.74 ± 23.54	10.07	3.89	231.11 ± 17.02	7.36	2.71
	700.00	696.42 ± 105.51	15.15	−0.51	711.56 ± 76.76	10.79	1.65	685.69 ± 52.24	7.62	−2.04
	1400.00	1371.79 ± 187.81	13.69	−2.02	1450.72 ± 161.20	11.11	3.62	1411.55 ± 129.21	9.15	0.83

^*Corresponds to ng/mL.