Table 5.
Parameters of calibration curve for each phytohormone: curve range, regression, weighting, correlation coefficient, limit of quantification (LOQ) and amount of each phytohormone present in blank Citrus sinensis samples.
| Analyte | Range (ng/g FW) | Curve* | R2 | LOQ (ng/g) | Amount in blank samples** (ng/g FW) | Matrix effect*** |
|---|---|---|---|---|---|---|
| IAA | 25–4000 | Y = 2.09382 + 0.0199062*X | 0.989 | 25 | 111.47 ± 17.95 | −16% |
| ABA | 20–2000 | Y = 3.463782 + 0.012662*X | 0.994 | 20 | 262.07 ± 6.71 | +2% |
| JA-Ile | 0.4–400 | Y = 0.176041 + 0.0702724*X | 0.994 | 0.4 | 1.91 ± 0.10 | +86% |
| JA | 12.5–2000 | Y = 0.74606 + 0.013142*X | 0.987 | 12.5 | 54.32 ± 9.43 | +147% |
| SA | 25–4000 | Y = 0.152181 + 0.00719421*X | 0.998 | 25.0 | 29.60 ± 6.35 | −4% |
| OPDA | 30–600 | Y = 1.41748 + 0.00743165*X | 0.981 | 30 | 85.15 ± 1.49 | −32% |
*
A weighting factor of 1/x2 was applied to all curves, except for OPDA, which used a factor of 1/x.
**
Values are average ± standard deviation. Concentrations represent the amount of each phytohormone in plant tissues (ng/g of fresh weight, FW), which is corresponding to the concentration (ng/mL) in the injection solution.
***
Values correspond to ((mmatrix/msolvent) −1)*100%.