Table 6.
Values of repeatability (accuracy and precision) obtained during the validation of the method for quantification of various phytohormones (ABA, IAA, JA-Ile, SA, JA, and OPDA) in leaves of Citrus sinensis.
| Expected conc. (ng/g FW)* | Repeatability n = 3 | % Recovery (mean + error) | Overall average of recovery | |||
|---|---|---|---|---|---|---|
| Mean ± SD | RSD (%) | Error (%) | ||||
| ABA | 70.00 | 73.13 ± 3.42 | 4.68 | 4.48 | 84.53 ± 13.04 | 82.77 ± 8.06 |
| 350.00 | 369.09 ± 15.06 | 4.08 | 5.46 | 75.63 ± 8.08 | ||
| 1400.00 | 1479.93 ± 73.67 | 4.98 | 5.71 | 88.15 ± 4.83 | ||
| IAA | 140.00 | 142.75 ± 9.55 | 6.69 | 1.97 | 55.65 ± 7.97 | 66.16 ± 8.51 |
| 1400.00 | 1488.05 ± 23.62 | 1.59 | 6.29 | 70.29 ± 7.42 | ||
| 2800.00 | 3023.73 ± 164.79 | 5.45 | 7.99 | 72.54 ± 5.86 | ||
| JA-Ile | 1.40 | 1.43 ± 0.17 | 11.87 | 2.38 | 39.74 ± 8.91 | 63.23 ± 10.90 |
| 70.00 | 69.96 ± 2.74 | 3.92 | −0.06 | 73.36 ± 7.59 | ||
| 280.00 | 300.15 ± 10.17 | 3.39 | 7.20 | 76.59 ± 5.82 | ||
| SA | 140.00 | 160.94 ± 2.88 | 1.79 | 14.96 | 61.16 ± 5.56 | 73.95 ± 5.19 |
| 1400.00 | 1611.94 ± 106.92 | 6.63 | 15.14 | 81.15 ± 7.58 | ||
| 2800.00 | 3205.07 ± 107.74 | 3.36 | 14.47 | 79.56 ± 3.75 | ||
| JA | 70.00 | 76.17 ± 4.97 | 6.52 | 8.82 | 63.61 ± 8.97 | 75.59 ± 7.40 |
| 700.00 | 747.05 ± 8.03 | 1.07 | 6.72 | 83.47 ± 7.73 | ||
| 1400.00 | 1496.15 ± 108.73 | 7.27 | 6.87 | 79.68 ± 4.81 | ||
| OPDA | 90.00 | 90.67 ± 7.34 | 8.10 | 0.74 | 66.19 ± 10.58 | 71.00 ± 10.70 |
| 300.00 | 303.39 ± 34.64 | 11.42 | 1.13 | 74.00 ± 9.77 | ||
| 420.00 | 450.81 ± 60.86 | 13.50 | 7.34 | 72.81 ± 6.57 | ||
*
Corresponding to the concentrations given in Validation in Citrus sinensis.