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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Mar 14;1840(7):2112–2122. doi: 10.1016/j.bbagen.2014.03.008

Fig. 5.

Fig. 5

mRNA levels and activity of MMPs in the radius and ulna. mRNA levels of MMP9 and MMP13 were analyzed in the radius and ulna of embryonic bone tissues (A) and E15.5 primary chondrocytes (B) of Chkb−/− and WT mice by quantitative real-time PCR. Data are means ± SEM from 3 littermates of each genotype; *: P < 0.05 compared to WT. (C): MMP9 activity was measured in primary chondrocytes from Chkb−/− and WT mice. Gel shows the presence of the proform and the mature form in the gelatin zymography assay. Minus = distilled water (negative control); plus = proteins from the chondrogenic cell line ATDC5 (positive control).

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