Fig. 8.

Biochemical analyses of primary chondrocytes. E15.5 bones were dissected free of soft tissue and primary chondrocytes were isolated for immunoblotting of CKβ (A), CK enzymatic activity (B), and quantification of choline metabolites (C, E) and phospholipids (D, F) by mass spectrometry. PC, phosphatidylcholine; PE, phosphatidylethanolamine; PS, phosphatidylserine; PI, phosphatidylinositol; SM, sphingomyelin. Data in B-F are means ± SEM from 3 littermates of each genotype; *: P < 0.05 compared to WT.