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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2014 May 27;1842(9):1596–1603. doi: 10.1016/j.bbadis.2014.05.016

Figure 1. Whole-cell current responses to glutamate receptor agonists and mEPSC activity are increased in LRRK2 transfected neurons.

Figure 1

(A, B) Steady state inward current responses to 100 µM AMPA at a holding potential of −55 mV were significantly larger in G2019S- and R1441C-expressing neurons, but not in K1906M-expressing neurons (* p<0.05 compared to vector-transfected neurons, ANOVA: 4 df, F-Ratio: 2.552; nVector = 24; nWT = 18; nG2019S = 27; nR1441C = 18 ; n1906M = 14). WT LRRK2-transfected neurons showed a trend towards increased AMPA responses. (C, D) Steady state inward current responses to 10 µM NMDA were significantly larger in G2019S and R1441C LRRK2 overexpressing neurons (* p<0.05 compared to vector-transfected neurons, ANOVA: 4 df, F-Ratio: 3.996) but were unaltered with overexpression of WT or K1906M LRRk2 (E) Raw traces from mEPSC recordings (holding potential −60 mV) showing increased mEPSC events per unit time in a R1441C LRRK2-transfected neuron (bottom) compared to the control vector-transfected neuron (top). (F) Composite data demonstrate increased mean mEPSC frequencies in neurons overexpressing mutant G2019S LRRK2 (n=10; p<0.05) or R1441C LRRK2 (n=10; p<0.05) and a modest trend towards increased mEPSC frequency in WT LRRK2 (n=14) compared to vector control (n=14) and K1906M LRRK2 (n=8; ANOVA: 4 df, F-Ratio: 2.654). (G) There was no difference in mean mEPSC amplitude among treatment groups (ANOVA: 4 df, F-Ratio: 0.545).

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