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. Author manuscript; available in PMC: 2015 Jan 18.
Published in final edited form as: Nat Commun. 2014 Jul 18;5:4456. doi: 10.1038/ncomms5456

Figure 1.

Figure 1

Images of GR single molecules in the nucleus of a live cell. HaloTMR-tagged GR molecules at selected time points (upper left corner) are presented from a 60 s movie. Projection of 85 successive timepoints from the movie defines the boundary of the nucleus (first frame). Color coding in subsequent panels indicates three separate segments of the movie illustrating a bound molecule (yellow - residence time of 10 s), a molecule that appears for only one timepoint (green) and a free molecule that diffuses in the plane of focus (red). Arrows indicate the molecule of interest. Red dotted line indicates the path of the diffusing molecule. Scale bar 1 µm.