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. 2014 Aug 26;5:449. doi: 10.3389/fmicb.2014.00449

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Copyright © 2014 López-Madrigal, Beltrà, Resurrección, Soto, Latorre, Moya and Gil.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genetic screening of selected loci involved in essential amino acid biosynthesis. The taxonomic assignation of the amplified sequences is indicated by black (betaproteobacteria) and red (gammaproteobacteria) circles. Empty circles represent pseudogenes. The species analyzed in this work appear in bold. P. citri and P. avenae endosymbionts are shown for comparison. Cladogram topology represents the evolutionary relationships between insect lineages (based on Hardy et al., 2008; Gruwell et al., 2010; this work). Marks on the branches indicate the infection by the ancestor of “Ca. Tremblaya” (β) and different lineages of gammaproteobacteria. γ1 corresponds to “Ca. Moranella endobia.” γ2–4 correspond to non-monophyletic gammaproteobacteria, based in our phylogenetic analyses.