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. 2014 Aug 26;5:449. doi: 10.3389/fmicb.2014.00449

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Copyright © 2014 López-Madrigal, Beltrà, Resurrección, Soto, Latorre, Moya and Gil.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Endosymbionts survey in P. longispinus. Left panel: Restriction maps for the four putatively present 16S rDNA sequences, identified by their accession numbers in GenBank, i.e., “Ca. Tremblaya princeps” (JN182336) and the sequences of gamma-proteobacterial origin identified by us (KF742539), Duron et al. (2008) (EU727120), and Rosenblueth et al. (2012) (HQ893843). RsaI restriction sites and expected fragments length are shown. Position numbers refer to the E. coli homolog. Right panel: RFLP results. Lanes 1 and 4, Molecular Weight Marker; lane 2, undigested amplicon; line 3, amplicon digested with RsaI. A band around 313 bp (labeled with an asterisk) is indicative of the presence of the KF742539 sequence (this work). Absence of a band around 129 bp rules out the presence of EU727120 and HQ893843.