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. 2014 Aug 11;2014:547480. doi: 10.1155/2014/547480

Figure 1.

Figure 1

Influence of ticlopidine on intact HUVEC and COS-7 cells transfected with an expression vector encoding human NTPDase1. The substrate (ATP or ADP) was added together with ticlopidine, both at 100 μM. Relative activities are expressed as the mean ± SD of 3 independent experiments with confluent cells (HUVEC from three different donors at passage 2), each performed in triplicate; mean cell number in one well was in the order of 250,000. The activity (without ticlopidine, which was set at 100%) with the substrate ATP corresponded to 2.5 ± 0.12 and 16.7 ± 0.8 nmol Pi·min−1 ·well−1 for HUVEC and transfected COS-7 cells, respectively, and with ADP to 3.5 ± 0.17 and 9.1 ± 0.45 nmol Pi·min−1 ·well−1 for HUVEC and transfected COS-7, respectively. Data are presented as the mean ± SD of 3 experiments carried out in triplicate. *P = 0.04; **P = 0.0003; ***P = 0.0001.