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. 2012 Jun 6;41(3):1139–1147. doi: 10.3892/ijo.2012.1508

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Copyright © 2012, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Quantitative real-time RT-PCR analysis of mRNA expression in ovarian tumors. Overexpression in cancer tissues was defined as 10% higher expression than the expression in normal tissue, excluding the testis. Eighteen cDNA samples composed of 16 ovarian tumor specimens, one normal ovarian tissue and one testis tissue sample were analyzed by real-time RT-PCR. mRNA levels were expressed as percentage differences relative to GAPDH (internal standard) and the levels in normal testis (calibrator).