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The mRNA expression of KP-OVA-52 in cancer cell lines. Cancer cell lines were treated for 5 days with or without 1 μM ADC. RT-PCR amplifications were performed with primers specific to the KP-OVA-52 gene. GAPDH was used as a loading control for RT-PCR. The ten cell lines used were nine KP-OVA-52 non-expressing cell lines (SNU-C1, SK-LC-5, SK-LC-14, NCI-H189, NCI-H82, SNU-C2A, NCI-H23, MCF-7 and NCI-H146) and expressing cell line (SK-OV-3).
