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. Author manuscript; available in PMC: 2015 Sep 8.
Published in final edited form as: Vaccine. 2014 Jul 26;32(40):5212–5220. doi: 10.1016/j.vaccine.2014.07.050

Figure 1. Mice immunized with mAb-iFt-pulsed BMDCs are better protected against a lethal Ft LVS challenge.

Figure 1

BMDCs pulsed with medium, iFt, or mAb-iFt (10 iFt/cell) were washed with PBS. Pulsed cells (3 × 106 cells/dose) were then suspended in 30 µl of PBS and administered to C57BL/6 mice (n=6) i.n. as droplets in alternating nares. Mice were primed and boosted with the Ag-pulsed BMDCs on days 0 and 14. Two weeks (A) and 4 weeks (B) post-boost, mice were challenged i.n. with 4 × LD50 (2 × 104 CFU) of Ft LVS in a volume of 20 µl of PBS. Survival was monitored for 28 days post-infection. The vehicle control groups received PBS only. Data presented are from a single experiment containing 6 mice per group. Each figure depicts results from a single experiment. The figure in panel A is representative of four independent experiments. The figure in panel B is representative of two independent experiments. In the case of panel A, the p value for the difference between iFt versus PBS is p=0.0045 and for iFt versus mAb-iFt 0.018. In the case of panel B, the p value for the difference between iFt versus PBS is p=0.0006 and for iFt versus mAb-iFt 0.2108.