Table 5.
Clozapine and valproate (VPA) co-administration reverses the methionine-induced downregulation of frontal cortex GAD67 expression in a methionine-induced epigenetic mouse model of schizophrenia
Treatments | GAD67 protein (vs β actin) |
---|---|
Methionine | 0.15 ± 0.020* |
Methionine + clozapine | 0.19 ± 0.015 |
Methionine + clozapine + VPA | 0.35 ± 0.032** |
Methionine + VPA | 0.29 ± 0.070 |
VEH | 0.38 ± 0.030 |
GAD67 protein levels (OD ratio of GAD67 to β actin) were determined by Western blot analyses in the frontal cortex of mice treated with methionine (5.2 mmol/kg s.c./twice a day/for 7 days)
VEH, VPA (70 mg/kg), clozapine (5 mg/kg) were administered to mice s.c./twice a day/for 3 days after methionine treatment termination.
Data represent the mean ± SE of 4 mice
P<0.01 for methionine vs VEH
P<0.01 for methionine vs methionine + clozapine + VPA