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Effect of AdoHcy antibody concentration on fluorescence polarization of AdoHcy tracer. Here 20-μl AdoHcy antibody dilutions were added to 30 μl tracer. FP was measured 30 min postaddition. Final dilution factors for anti-AdoHcy antibody in 50 μl are indicated; that of tracer is 160. Data are representative of two independent experiments.
