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. Author manuscript; available in PMC: 2014 Aug 26.
Published in final edited form as: Anal Biochem. 2007 Sep 29;373(2):296–306. doi: 10.1016/j.ab.2007.09.025

Fig. 3.

Fig. 3

Mock methyltransferase assays using increasing AdoHcy concentrations and proportionally decreasing AdoMet concentrations. The assay was performed in 30 μl with AdoHcy at the indicated concentrations and total AdoHcy + AdoMet concentrations of 1 μM (◆), 3 μM (▼), 10 μM (▲), and 30 μM (■). (A) The AdoHcy/AdoMet mixtures were combined with 20 μl antibody/tracer complex, and FP was measured 2 h postaddition. (B) Differential mP was determined by subtracting mP values at 0 AdoHcy from mP values at the indicated AdoHcy concentrations.