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Time course of COMT activity assay. Reactions were performed for the indicated lengths of time using 0.33 μg COMT enzyme and 3 μM AdoMet in the presence (■) or absence (▲) of 250 μM DBA substrate. Reactions were initiated with the addition of AdoMet, and all reactions were terminated simultaneously with the addition of 10 mM EDTA in the 20-μl antibody/tracer complex solution. FP was measured 1 h after termination of the reaction.
