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. Author manuscript; available in PMC: 2014 Aug 26.
Published in final edited form as: Anal Biochem. 2007 Sep 29;373(2):296–306. doi: 10.1016/j.ab.2007.09.025

Fig. 7.

Fig. 7

Inhibition curve using a known COMT inhibitor. Reactions were performed using 0.33 μg COMT enzyme, 3 μM AdoMet, and 250 μM DBA substrate with indicated concentrations of Ro 41-0960 inhibitor. COMT and Ro 41-0960 were preincubated together for 5 min, and then the reactions were initiated by the addition of AdoMet. Reactions were terminated with 10 mM EDTA in the 20-μl antibody/tracer complex solution. FP was determined 1 h after terminating the reaction. The average IC50 value from three independent experiments was 12 nM.