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. 2014 Sep;144(3):257–261. doi: 10.1085/jgp.201411243

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© 2014 Ji et al.

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Figure 4. — F⁻ uptake by ΔFluc E. coli cells as a function of pH or F⁻ concentration. (A) Ratio of internal and external [F⁻] with varying pH. The dashed line has a slope of 1, as predicted by Eq. 1. Inset plots data directly against medium pH. (B) Ratio of internal and external F⁻. The solid line is a fit to the data with slope of 54, corresponding to a transmembrane pH differential of 1.7 units. F⁻ uptake was determined as in Fig 3. Each point shows mean (±SEM) of three independent measurements. Internal pH was assumed to remain constant at 7.0.