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. Author manuscript; available in PMC: 2014 Aug 26.
Published in final edited form as: Nat Biotechnol. 2012 Mar 25;30(5):453–459. doi: 10.1038/nbt.2158

Figure 3. Bdnf upregulation increases neuronal outgrowth.

Figure 3

(A–B) Immunocytochemistry images of hippocampal neurospheres treated with either control siRNA (A) or Bdnf-AS siRNA (B) 3 d post-platting. (C–D) Immunocytochemistry images of neuronal maturation and neurite outgrowth in hippocampal neurospheres treated with either control siRNA (C) or Bdnf-AS siRNA (D) 7 d post-plating. Treatment of cells with siRNA targeting the Bdnf-AS transcript resulted in increased neuronal cell number as well as increase in neurite outgrowth and maturation, both at 3d or 7d post-plating neurospheres. B-tubulin III stained red, GFAP stained green and DAPI stained blue.