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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Crit Care Med. 2014 Mar;42(3):e189–e199. doi: 10.1097/CCM.0000000000000097

Figure 2. Tys maintains S1PR1 protein expression compared to other agonists.

Figure 2

Fig. A, B: HPAEC were stimulated with vehicle control (c), S1P, FTY720, Tys, 1R, p-FTY720 (each at 1 µM), or SEW (10 µM) for 4 h. S1PR1 expression level was detected by Western blot. A, representative western blot; B, Bar graph represents pooled densitometry from 3 independent experiments. Fig. C, D: HPAEC were pretreated with 20 µM MG132 (MG) (a proteasome inhibitor) for 2 h, and then stimulated with vehicle control (C), Tys, 1R, or p-FTY720 (1 µM) for 4h. S1PR1 expression level was detected by Western blot. C, representative western blot; D, Bar graph represents pooled densitometry from 3 independent experiments. *, p<0.01 vs. control (C); #, p<0.01 compared to 1R without MG; &, p<0.05 compared to p-FTY720 without MG.