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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Crit Care Med. 2014 Mar;42(3):e189–e199. doi: 10.1097/CCM.0000000000000097

Figure 5. S1P and FTY720, but not Tys, induce internalization of S1PR1.

Figure 5

Confluent HLMVEC grown on glass bottom culture dishes were stimulated with vehicle control, Tys, FTY720, or S1P (1 µM) for 2 h. Cells were then fixed and immunostained with S1PR1 antibody per standard protocol. Arrows indicate that S1PR1 localizes to the cell periphery in control and Tys-treated cells, while S1P and FTY720 induce internalization of the receptor. Representative figures from 3 independent experiments are shown.