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. 2014 Aug 25;211(9):1857–1874. doi: 10.1084/jem.20130791

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© 2014 Tanaka et al.

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Figure 1. — A novel isoform of Sox5 is expressed in IL-6–stimulated CD4⁺ T cells. (A) Alignments between Sox5t and NCBI RNA reference sequences collection (RefSeq Genes) and mouse mRNAs in GenBank are shown. (B, top) Schema of the coding region of Sox5t and l-Sox5 (AJ010604.1). The locations of the primers used in qPCR analysis (Sox5t primer,l-Sox5 primer, and Sox5 HMG primer) are shown. (bottom) Sequence comparison of Sox5t and l-Sox5. Aligned are the first 360 nucleotides of Sox5t and 243 nucleotides of l-sox5 and the predicted amino acid sequence for Sox5t and l-Sox5. (C) Schematic comparison of Sox5t and l-Sox5. (D) The expression levels of Sox5t, l-Sox5, and Sox5 HMG in various tissues and CD4⁺ T cells. qPCR was performed by using Sox5t, l-Sox5, and Sox5 HMG primers indicated in B. Shown are means ± SD. Data are compiled from three independent experiments. (E) Naive CD4⁺ T cells from CD4^cre Stat3^fl/fl mice or Stat3^fl/fl mice were stimulated under either neutral conditions, IL-6 conditions, or Th17 conditions for 4 d, and subjected to Western blot analysis for Sox5, c-Maf, and Hsp90 (as a control). Data are representative of three independent experiments.