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. 2014 Aug 25;211(9):1793–1805. doi: 10.1084/jem.20131902

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© 2014 Marshall et al.

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Figure 1. — In vitro characterization of CR2-CD59. (A) Flow cytometric analysis of CR2-CD59 binding to C3-opsonized CHO cells. Antibody-sensitized CHO cells were incubated with C6^−/− mouse serum followed by incubation with CR2-CD59 (thick black trace) or PBS (dark gray trace). CR2-CD59 was also incubated with unopsonized cells, either without antibody (light gray trace) or without serum (thin black trace). Shown is a representative of 3 separate experiments. (B) CR2-CD59 inhibition of complement-mediated RBC lysis. Antibody-sensitized chicken RBCs were incubated with either CR2-Crry or CR2-CD59 in the presence of mouse serum. (C) Effect of CR2-CD59 on C3 activation. Activated zymosan particles were incubated with mouse serum and increasing doses of CR2-Crry or CR2-CD59, and C3 deposition on particles detected by flow cytometry. Data are presented as mean ± SEM (n = 2–3) and are representative of 2–4 independent experiments.