Figure 3.

Quantity versus quality of RSVepitope-specific CD8⁺ T cell responses in mice infected with RSV_wt, RSV-M2_I90T, or RSV-M_N191S. A, Frequency and cytokine production by K^dM2_82–90-specific CD8⁺ T cells in the lung following infection with RSV_wt, RSV-M2_I90T, or RSV-M_N191S. Parallel samples were stained with tetramer and incubated with M2_82–90 peptide for 5 h in the presence of the transport inhibitor monensin. B, Fraction of K^dM2_82–90-specific cells capable of producing cytokine following M2_82–90 peptide stimulation. C, MFI of IFN-γ–positive cells following peptide stimulation with M2_82–90 following group gating on cells producing IFN-γ. D, Frequency and cytokine production by D^bM_187–195 epitope-specific CD8⁺ T cells following infection. E, Fraction of D^bM_187–195-specific cells capable of producing cytokine following M_187–195 peptide stimulation. F, MFI of IFN-γ–positive cells following peptide stimulation with M_187–195. *p < 0.05 compared with RSV_wt; ***p < 0.001 compared with RSV_wt. Data are representative of two experiments with four to five mice/group and error bars represent the SEM. ns, no significance.