Fig. 1.

Relative gene transcript levels of PlOS quantified in P. lunatus plants treated with (i) water (control), (ii) 0.1mM JA, (iii) four T. urticae (water+4Tu), or (iv) 0.1mM JA with four T. urticae mites (0.1mM JA+4Tu). (A) Inoculation of four adult female T. urticae on plants was done immediately following JA-treatment and mites had been feeding for 48h, and (B) inoculation of four adult female T. urticae was done 7 days after incubation with water or 0.1mM JA and mites had been feeding for 48h. Values are the mean (± SE) of three to four biological replicates, different letters above bars indicate significant differences in transcript levels between treatments (ANOVA followed by Fisher’s LSD test, α=0.05). PlOS transcript levels were normalized to the normalization factor obtained from geometrically averaging the Ct values of the two reference genes PlACT1 and PlNMP1 for each sample. Baseline represents transcript level in control plants.