Fig. 4.
Multivariate data analysis by PLS-DA and corresponding loading plot of targeted volatiles of P. lunatus plants exposed to (i) water (control, W), (ii) 0.1mM JA (J), (iii) water and four T. urticae spider mites (WTu), or combined treatment (iv) 0.1mM JA with immediate application of four T. urticae (JTu). (A) PLS-DA score plot showing the ordination of the samples according to the first two PLS components based on the quantitative values of volatiles between different treatments. Explained variance by first and second PLS components is given in brackets. Loading plot (B) shows the contribution of each volatile to the discrimination between treatments using the first two PLS components. Numbers represent: 1, (E)-2-hexenal; 2, (Z)-3-hexen-1-ol; 3, (Z)-3-hexen-1-ol acetate; 4, (E)-β-ocimene; 5, linalool; 6 (E)-4,8-dimethyl-1,3,7-nonatriene [(E)-DMNT]; 7, methyl salicylate (MeSA); 8, indole; 9, β-caryophyllene; 10, (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene [(E,E)-TMTT]. Squares represent the four treatments (labelled W, WTu, J, and JTu).