Fig. 6.

Complementation of pxa1/pxa2Δ by HvABCD1. β-oxidation of different fatty acids by intact yeast cells. Cells grown in oleate medium were incubated with 1-¹⁴C-labelled fatty acids, followed by β-oxidation activity measurements. Strains are: wild type (BJ1991), pxa1/pxa2Δ mutant cells transformed with vector lacking the insert, or HvABCD1. fox1Δ is defective in acyl-CoA oxidase (the first committed step of β-oxidation) and serves as a negative control. Results are presented as a percentage relative to the rate of oxidation by wild-type cells for each fatty acid and are means ±SD of three independent experiments.