Fig. 7.

Expression analyses of CsCUC3 and CsSTM during fruit spine development in cucumber. (A) Semi-quantitative RT-PCR of CsCUC3 in pericarps of WT and tbh mutant. (B–F) Gene expression patterns as detected by in situ hybridization. CsCUC3 was expressed in the epidermis and nucleus of spine cells in developing fruit spines of WT (arrows in B and C), but was undetectable in the tbh mutant (D, E). (F) Transcript accumulation of CsSTM was localized in the epidermis and nucleus of spine cells (arrows), and was strongly induced at the junction of the fruit base and wart during fruit base expansion (asteroids) in WT cucumber. Bars, 200 µm. (This figure is available in colour at JXB online.)